姓　名：张智红

职　称：教授

导师类别：博士生导师、硕士生导师

E-mail：zhzhang@hainanu.edu.cn

一、个人基本情况

  张智红，医学博士，无党派人士，海南大学生命健康学院教授。国家重点研发计划重点专项负责人、国家杰出青年科学基金获得者。

二、教育和工作经历

  1999年华中科技大学同济医学院（原同济医科大学）免疫学专业毕业，获医学硕士学位。2003年华中科技大学生物医学工程专业毕业，获医学博士学位。自1999年7月，在华中科技大学生命科学与技术学院和武汉光电国家研究中心工作，历任讲师（2000.7）、副教授（2004.11）、教授（2008.12）。2002年4月作为访问学者赴美国宾夕法尼亚大学医学院Dr. Britton Chance实验室进行为期一年的肿瘤代谢成像合作研究。2007年-2011年期间，曾多次访问加拿大多伦多大学/安大略癌症研究院Dr. Gang Zheng实验室，进行多肽-脂质纳米载体的合作研究。2022年11月在海南大学工作。

三、教学情况和研究生培养

  讲授本科生的《普通生物学》、《细胞生物学》和《生物医学工程前沿》，研究生的《医学实验动物模型》、《分子影像学》和《光学分子成像》等课程。

  已培养并毕业博士研究生28人，培养并毕业硕士研究生14人。其中，金红林博士（2012年毕业）获国家级青年项目（2020年），骆海明博士（2013年毕业）获国家级青年项目（2017年），余祥博士（2019年毕业）获第十届中国免疫学会学术奖“青年学者奖”（2021年）。

四、科研情况和研究方向

  近年来，针对免疫反应可视化研究的重大需求，致力于发展多分子并行检测、活体靶向标记和多层次光学成像新方法，建立的免疫光学成像新技术新方法已应用于肝脏、皮肤、脑等相关疾病模型的免疫应答可视化研究。在Nature Communications、Science Advances、Light: Science & Applications、eLife、Theranostics、Advanced Science、ACS Nano、Autophagy和Brain Behavior and Immunity等SCI期刊上发表第一或通讯作者（含并列）论文60多篇。授权中国发明专利21项。

  主要研究方向：

1）肝脏脉管-神经-免疫互作的介观三维成像

2）肿瘤免疫微环境时空变化的活体光学分子成像

3）慢性疾病的免疫调节与多模态成像可视化研究

五、主持的科研项目

1)国家自然科学基金重点项目，“光学成像可视化研究肝转移过程中肿瘤异质性与微环境的时空变化”，2024/01-2028/12，216万元

2)国家重点研发计划“变革性技术关键科学问题”重点专项, “完整肝脏三维结构与功能信息的精准介观测量”，2018/05-2023/04，2776万元

3)国家杰出青年科学基金，“肿瘤免疫光学分子成像”，2017/01-2021/12，350万元

4)国家自然科学基金重大研究计划重点支持项目，2015/01-2018/12，300万元

5)国家自然科学基金面上项目，2012/01-2015/12，55万元 ;

6)国家科技支撑计划课题，2012/01-2014/12，362万元

7)教育部“新世纪优秀人才支持计划”， 2009/01-2011/12，50万元

8)国家自然科学基金面上项目，2008/01-2010/12，38万元

六、荣誉和奖励

2006年获湖北省优秀博士论文《基于内源性和外源性荧光分子的肿瘤代谢成像》

2008年教育部“新世纪优秀人才支持计划”入选者

2008年获湖北省自然科学一等奖《用于药物筛选和药效评价的光学分子成像动态监测理论与方法研究》，第二完成人

2010年获国家自然科学二等奖《生物功能的飞秒激光光学成像机理研究》，第五完成人

2016年国家杰出青年科学基金获得者

七、学术兼职

  学术期刊Cellular & Molecular Immunology、European Journal of Nuclear Medicine and Molecular Imaging、iRadiology、Bioconjugate Chemistry、《生物化学与生物物理进展》和《中国免疫学杂志》编委会委员。国际光学工程学会（SPIE）Photonics West “Biophotonics and Immune Responses”会议的委员会成员（Program Committee Member）（会议地点：美国旧金山）。中国抗癌协会全国肿瘤精准治疗专委会常务委员、中国认知科学学会分子影像分会常务理事、中国老年学和老年医学学会老年病学分会肿瘤基础及转化研究专家委员会常委、中国电子学会生命电子学分会常务委员。

八、代表性成果（论文、专利、专著等）

Ø 代表性论文（¹并列第一作者；*通讯作者）

1) Zheng Liu¹, Zhan Fan¹, Jinxin Liu, Jialu Wang, Mengli Xu, Xinlin Li, Yilun Xu, Yafang Lu, Chenlu Han, Zhihong Zhang*. Melittin-carrying nanoparticle suppress T cell-driven immunity in a murine allergic dermatitis model. Advanced Science, 10, 2204184, 2023.

2) Songlin Huang¹, Borui Li¹, Zheng Liu¹, Mengli Xu, Dong Lin, Jiahong Hu, Dongjian Cao, Qi Pan, Jing Zhang, Jing Yuan, Qingming Luo*, Zhihong Zhang*. Three-dimensional mapping of hepatic lymphatic vessels and transcriptome profiling of lymphatic endothelial cells in healthy and diseased livers. Theranostics, 13(2): 639-658, 2023.

3) Junjie Wang¹, Xingzhou Peng¹, Jianshuang Wei, Yanfeng Dai, Songlin Huang, Deqiang Deng, Zhan Fan, Yuehong Leng, Jing Ai, Zhenzhen Cai, Maosong Qiu, Shizhen Chen, Xiaoquan Yang*, Zhihong Zhang*. In situ phagocyte-mediated deep tumor penetration assisted by ApoA-1 mimetic peptide-modified silicasome. Nano Today. 50: 101864,2023.

4) Qi Pan, Borui Li, Dong Lin, Ya-Ru Miao, Tao Luo, Tao Yue, Qingming Luo, An-Yuan Guo^*, Zhihong Zhang*. scLiverDB: a database of human and mouse liver transcriptome landscapes at single-cell resolution. Small Methods, 22014, 2023.

5) Hao Zheng¹, Songlin Huang¹, Jing Zhang, Ren Zhang, Jialu Wang, Jing Yuan, Anan Li, Xin Yang^*, Zhihong Zhang^*. C1M2: a universal algorithm for 3D instance segmentation, annotation, and quantification of irregular cells. Science China-Life Sciences. 2023.

6) Yanfeng Dai, Xiang Yu, Yuehong Leng, Xingzhou Peng, Junjie Wang, Yifan Zhao, Juan Chen, Zhihong Zhang*. Effective treatment of metastatic sentinel lymph nodes by dualtargeting melittin nanoparticles. Journal of Nanobiotechnology. 21: 254, 2023.

7) Jian Li¹, Lisen Lu¹, Kyle Binder, Jian Xiong, Lilin Ye, Yan H. Cheng, Sonia Majri-Morrison, Wei Lu, Jae Lee, Zhihong Zhang^*, Yuzhang Wu^*, Lixin Zheng^*, and Michael J. Lenardo^*. Mechanisms of antigen-induced reversal of CNS inflammation in experimental demyelinating disease. Science Advances, 2023.

8) Bolei Dai¹, Ren Zhang¹, Shuhong Qi*, Lei Liu, Xian Zhang, Deqiang Deng, Jie Zhang, Yilun Xu, Fanxuan Liu, Zheng Liu, Qingming Luo, Zhihong Zhang*. Intravital molecular imaging reveals that ROS-caspase-3-GSDME-induced cell punching enhances humoral immunotherapy targeting intracellular tumor antigens. Theranostics, 12(17): 7603-7623, 2022.

9) Zheng Liu¹, Mengli Xu¹, Songlin Huang¹, Qi Pan, Chong Liu, Fanxin Zeng, Zhan Fan, Yafang Lu, Jialu Wang, Jinxin Liu, Xinlin Li, Qingming Luo*, Zhihong Zhang*. Mesoscale visualization of three-dimensional microvascular architecture and immunocyte distribution in intact mouse liver lobes. Theranostics 2022; 12(12):5418-5433. (封面文章)

10) Deqiang Deng¹, Bolei Dai¹, Jianshuang Wei, Xuenan Yuan, Xiaoquan Yang, Shuhong Qi*, Zhihong Zhang*. A drawer-type abdominal window with an acrylic/resin coverslip enables long-term intravital fluorescence/photoacoustic imaging of liver. Nanophotonics, 10(12): 3369–3381, 2021.

11) Lei Liu, Bolei Dai, Ruixue Li, Zheng Liu, Zhihong Zhang*. Intravital molecular imaging reveals the restrained capacity of CTLs in the killing of tumor cells in the liver. Theranostics, 11(1): 194-208, 2021.

12) Vasiliki Staikopoulos¹, Sha Qiao¹, Jiajun Liu, Xianlin Song, Xiaoqun Yang, Qingming Luo, Mark R Hutchinson, Zhihong Zhang*. Graded peripheral nerve injury creates proportional mechanical allodynia to the progression and severity of microglial activity within the spinal cord of male mice. Brain Behavior and Immunity. 91:568-577, 2021.

13) Haiming Luo^1,*, Lisen Lu¹, Ni Liu, Qingqing Li, Xiaoquan Yang, Zhihong Zhang*. Curcumin loaded sub-30 nm targeting therapeutic lipid nanoparticles for synergistically blocking nasopharyngeal cancer growth and metastasis. Journal of Nanobiotechnology, 19:224, 2021.

14) Yanfeng Dai¹, Xiang Yu¹, Jianshuang Wei¹, Fanxin Zeng, Yiran Li, Xiaoquan Yang, Qingming Luo*, Zhihong Zhang*. Metastatic status of sentinel lymph nodes in breast cancer determined with photoacoustic microscopy via dual-targeting nanoparticles. Light: Science & Applications. 9:164, 2020.

15) Lisen Lu¹, Shuhong Qi¹, Yuzhou Chen, Haiming Luo, Songlin Huang, Xiang Yu, Qingming Luo, Zhihong Zhang*. Targeted immunomodulation of inflammatory monocytes across the blood-brain barrier by curcumin-loaded nanoparticles delays the progression of experimental autoimmune encephalomyelitis. Biomaterials. 245:119987, 2020.

16) Xiang Yu¹, Yanfeng Dai¹, Yifan Zhao, Shuhong Qi, Lei Liu, Qingming Luo, Zhihong Zhang*. Melittin-lipid nanoparticles target to lymph node and elicit a systemic anti-tumor immune response. Nature Communications. 11, 1110, 2020.  

17) Shuhong Qi¹, Lisen Lu¹, Feifan Zhou, Yuzhou Chen, Mengli Xu, Lu Chen, Xiang Yu, Wei R. Chen*, Zhihong Zhang*. Neutrophil infiltration and whole-cell vaccine elicited by N-dihydrogalactochitosan combined with NIR phototherapy to enhance antitumor immune response and T cell immune memory. Theranostics. 10(4): 1814-1832, 2020.  

18) Qiaoya Lin¹, Deqiang Deng¹, Xianlin Song, Bolei Dai, Xiaoquan Yang, Qingming Luo*, Zhihong Zhang*. Self-Assembled “Off/On” Nanopomegranate for in vivo photoacoustic and fluorescence imaging: strategic arrangement of kupffer cells in mouse hepatic lobules. ACS Nano. 13 (2), 1526–1537, 2019.

19) Xiang Yu, Lu Chen, Jianqiao Liu, Bolei Dai, Guoqiang Xu, Guanxin Shen, Qingming Luo*, Zhihong Zhang*. Immune modulation of liver sinusoidal endothelial cells by melittin nanoparticles suppresses liver metastasis. Nature Communications. 10:574, 2019.  

20) Sha Qiao¹, Yuan Qian¹, Guoqiang Xu, Qingming Luo, Zhihong Zhang*. Long-term characterization of activated microglia/macrophages facilitating the development of experimental brain metastasis through intravital microscopic imaging. Journal of Neuroinflammation. 16:4, 1-14, 2019.

21) Zheng Liu, Fei Yang, Hao Zheng, Zhan Fan, Sha Qiao, Lei Liu, Juan Tao, Qingming Luo^*, Zhihong Zhang^*. Visualization of T cell-regulated monocyte clusters mediating keratinocyte death in acquired cutaneous immunity. Journal of Investigative Dermatology. 138: 1328-1337, 2018. (封面文章)

22) Yuan Qian¹, Sha Qiao¹, Yanfeng Dai, Guoqiang Xu, Bolei Dai, Lisen Lu, Xiang Yu, Qingming Luo, Zhihong Zhang*. Molecular-targeted immunotherapeutic strategy for melanoma via dual-Targeting nanoparticles delivering small interfering RNA to tumor-associated macrophages. ACS Nano, 11(9), 9536−9549, 2017.

23) Liang Wang, Lei Liu, Lingsong Qin, Qingming Luo, Zhihong Zhang*. Visualization of reticulophagy in living cells using an endoplasmic reticulum-targeted p62 mutant. Science China-Life Sciences. 60(4):333-344, 2017. (封面文章)  

24) Shuhong Qi¹, Hui Li¹, Lisen Lu, Zhongyang Qi, Lei Liu, Lu Chen, Guanxin Shen, Ling Fu, Qingming Luo*, Zhihong Zhang*. Long-term intravital imaging of the multicolor-coded tumor microenvironment during combination immunotherapy. eLife, 5: e14756, 2016.

25) Honglin Jin, Yuan Qian, Yanfeng Dai, Sha Qiao, Chuan Huang, Lisen Lu, Qingming Luo, Jing Chen*, Zhihong Zhang*. Magnetic enrichment of dendritic cell vaccine in lymph node with fluorescent-magnetic nanoparticles enhanced cancer immunotherapy. Theranostics, 6(11): 2000-2014, 2016.

26) Fei Yang¹, Shun Liu¹, Xiuli Liu¹, Lei Liu¹, Meijie Luo, Shuhong Qi, Guoqiang Xu, Sha Qiao, Xiaohua Lv, Xiangning Li, Ling Fu, Qingming Luo*, Zhihong Zhang*. In vivo visualization of tumor antigen-containing microparticles generated in fluorescent-protein-elicited immunity. Theranostics. 6(9):1453-1466, 2016.

27) Yuan Qian¹, Honglin Jin¹, Sha Qiao, Yanfeng Dai, Chuan Huang, Lisen Lu, Qingming Luo*, Zhihong Zhang*. Targeting dendritic cells in lymph node with an antigen peptide-based nanovaccine for cancer immunotherapy. Biomaterials. 98, 171-183, 2016.

28) Haiming Luo, Lisen Lu, Fei Yang, Liang Wang, Xiaoquan Yang, Qingming Luo, Zhihong Zhang*. Nasopharyngeal cancer-specific therapy based on fusion peptide-functionalized

29) Meijie Luo, Zhihong Zhang*, Hui Li, Sha Qiao, Zheng Liu, Ling Fu, Guanxin Shen, Qingming Luo*. Multi-scale optical imaging of the delayed type hypersensitivity reaction attenuated by rapamycin. Theranostics. 4(2): 201-214, 2014.

30) Chuan Huang, Honglin Jin, Yuan Qian, Shuhong Qi, Haiming Luo, Qingming Luo, Zhihong Zhang*. Hybrid melittin cytolytic peptide-driven ultrasmall lipid nanoparticles block melanoma growth in vivo. ACS Nano, 7(7): 5791–5800, 2013.  

31) Ying Zheng^#, Yanyan Liu^#, Honglin Jin^#, Shaotao Pan, Yuan Qian, Chuan Huang, Yixin Zeng, Qingming Luo, Musheng Zeng*, Zhihong Zhang*. Scavenger receptor B1 is a potential biomarker of human nasopharyngeal carcinoma and its growth is inhibited by HDL-mimetic nanoparticles. Theranostics. 3(7):477-486, 2013.

32) Liang Wang¹, Min Chen¹, Jie Yang, Zhihong Zhang*. LC3 fluorescent puncta in autophagosomes or in protein aggregates can be distinguished by FRAP analysis in living cells. Autophagy. 9(5): 756-769, 2013.

33) Ting Su, Shaotao Pan, Qingming Luo, Zhihong Zhang*. Monitoring of dual bio-molecular events using FRET biosensors based on mTagBFP/sfGFP and mVenus/mKOκ fluorescent protein pairs. Biosensors and Bioelectronics. 46: 97-101, 2013.

34) Haiming Luo, Jiyun Shi, Honglin Jin, Di Fan, Lisen Lu, Fan Wang*, Zhihong Zhang*. An ¹²⁵I-labeled octavalent peptide fluorescent nanoprobe for tumor-homing imaging in vivo. Biomaterials. 33 (19): 4843-4850, 2012.

35) Zhihong Zhang¹, Juan Chen¹, Lili Ding, Honglin Jin, Jonathan F. Lovell, Ian Corbin, Weiguo Cao, Pui-Chi Lo, Mi Yang, Ming-Sound Tsao, Qingming Luo, Gang Zheng*, HDL-mimicking peptide-lipid nanoparticles with improved tumor targeting. Small. 6(3): 430-437, 2010.

36) Zhihong Zhang, Weiguo Cao, Honglin Jin, Jonathan F. Lovell, Mi Yang, Lili Ding, Juan Chen, Ian Corbin, Qingming Luo, Gang Zheng*. Biomimetic nanocarrier for direct cytosolic drug delivery. Angewandte Chemie International Edition. 48(48): 9171-9175, 2009.

37) Zhihong Zhang, Hui Li, Qian Liu, Lanlan Zhou, Ming Zhang, Qingming Luo*, Jerry Glickson, Britton Chance, Gang Zheng. Metabolic imaging of tumors using intrinsic and extrinsic fluorescent markers. Biosensors & Bioelectronics. 20(3): 643-650, 2004.

38) Zhihong Zhang, Dana Blessington, Theresa M. Busch, Jerry Glickson, Qingming Luo*, Britton Chance and Gang Zheng. Redox ratio of mitochondria as an indicator for the response of Photodynamic Therapy. Journal of Biomedical Optics. 9(4): 772-778, 2004.

Ø 专著与综述

1) Mengli Xu¹, Zheng Liu¹, Xinlin Li, Xinru Wang, Xuenan Yuan, Chenlu Han, Zhihong Zhang*. Three-dimensional structure of liver vessels and spatial distribution of hepatic immune cells. Journal of Innovative Optical Health Sciences. 2330006, 2023.

2) Xuenan Yuan, Xiang Yu, Bolei Dai, Zhihong Zhang*. “Advances in intravital imaging of liver immunity using optical microscopy and labeling methods”. iRADIOLOGY, 1(1): 61-77, 2023.

3) Xingzhou Peng¹, Junjie Wang¹, Feifan Zhou, Qian Liu*, Zhihong Zhang*.Nanoparticle-based approaches to target the lymphatic system for antitumor treatment. Cellular and Molecular Life Sciences, 78(12):5139-5161, 2021.

4) 林巧雅, 黄松林, 骆清铭, 张智红*. 肝脏免疫的活体显微光学成像研究进展. 生物化学与生物物理进展. 44(12): 1056~1065, 2017. (封面文章)

5) Zhihong Zhang, Qingming Luo, Chapter 1: Fluorescent Protein Labeling Techniques. “ADVANCES IN MOLECULAR BIOPHOTONICS”. ISBN 978-3-11-030438-1, Ó2017 Shanghai Jiao Tong University Press and Walter de Gruyter GmbH, Berlin/Boston

6) 张智红、韩岩梅，免疫学新技术进展及发展趋势，《2014-2015免疫学学科发展报告》第十八章，书号：ISBN 978-7-5046-7093-9/R·1876，中国科学技术出版社，2016年4月出版。

7) 张智红、骆清铭，新型荧光蛋白标记技术，《生物分子光子学研究前沿》第一章，书号：ISBN978-7-313-11756-4，上海交通大学出版社，2014年出版.

8) 张智红、吕群燕、骆清铭、程京、曹雪涛，细胞功能的可视化及信息整合分析——第69次“双清论坛”综述，中国科学基金，26(4): 195-198, 2012.

9) 骆清铭、张智红，免疫光子学进展. 光学学报. 31: 900114, 2011.